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. 2010 Sep 21;42A(4):219–227. doi: 10.1152/physiolgenomics.00137.2010

Fig. 1.

Fig. 1.

Polymorphic variants in zinc transporter 2 (ZnT2) result in mislocalization of the protein. A: representative immunoblot (IB) of crude membrane proteins (50 μg protein/lane) from cells transfected with pcDNA3.1 (Mock), or cells expressing wild-type ZnT2-hemagglutinin (HA) fusion protein (ZnT2), single nucleotide polymorphism (SNP)1-HA fusion protein (SNP1), or SNP2-HA fusion protein (SNP2) detected with HA antibody. *Nonspecific protein recognized by the anti-HA antibody. B: localization of wild-type ZnT2-HA (A–C), SNP1-HA (D–F), or SNP2-HA (G–I) in transfected HC11 cells examined using confocal microscopy. ZnT2 was detected with Alexa 488-conjugated anti-HA antibody (green; A, D, and G), and the nucleus was stained with TOPRO nucleic acid dye (red; B, E, and H).