Fig. 7.

Reactive oxygen species (ROS) levels were significantly increased in cells ectopically expressing SNP2. ROS level was assessed using dichlorofluorescein (DCF)-HA fluorescence in cells transfected to express each SNP and compared with cells generated to overexpress wild-type ZnT2. To validate our assay, H₂O₂ (100 μM) was used as a positive control. Data represent mean fluorescence ± SD (n = 8 samples/group). Means with different letters are significantly different as analyzed by 1-way analysis of variance followed by a Tukey posttest (P < 0.05).