Fig. 5. Combinations of clemizole with SCH503034 significantly reduce the frequency of phenotypic resistance.
Huh7 cells electroporated de novo with a genotype 1b subgenomic HCV replicon were treated in duplicates with various concentrations of clemizole and SCH503034, either alone or in combinations, in the presence of G418 selection. Following 3 weeks of treatment, plates were stained with crystal violet.
A- Representative plates
B- Colonies were counted using Image-J (NIH) and their number was used to calculate the frequency of resistance (number of colonies/number of input cells).