Skip to main content
. Author manuscript; available in PMC: 2010 Dec 22.
Published in final edited form as: Eur J Immunol. 2008 Sep;38(9):2438–2450. doi: 10.1002/eji.200838289

Figure 1. Gene expression analyzed using Affymetrix chips representing 35,000 oligonucleotides.

Figure 1

2-DG affects only a subset of genes in stimulated CD8+ effector T cells. CD8+ effector T cells were stimulated for 12 hours with anti-CD3 and anti-CD28 mAb-coated beads in the presence or absence of 50 mM 2-DG. Control (Ctrl), unstimulated; stimulated (Stim); stimulated in the presence of 50 mM 2-DG (2-DG). Genes affected by 2-DG treatment include IFN-γ (A), several cytokines including GM-CSF, IL-3, IL-9, IL-10 (B) but not MIP-1β and IL-2 (C). Cytolytic and proliferative genes like perforin, granzyme B (D) and cyclin D2 (E) were also highly affected by 2-DG treatment as compared to unmodulated control genes like γ-tubulin and TCR-β (F). Individual hybridizations were normalized using dChip software, then averaged. Data represent the average + SD of two independent experiments.