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. Author manuscript; available in PMC: 2010 Dec 22.
Published in final edited form as: Eur J Immunol. 2008 Sep;38(9):2438–2450. doi: 10.1002/eji.200838289

Figure 4. 2-DG inhibits cell cycle progression of stimulated CD8+ effector T cells.

Figure 4

A. Quantitative RT-PCR of cyclin D2 mRNA levels in cells treated anti-CD3 and anti-CD28 mAb-coated beads in the presence or absence of 2-DG. Data represent the average of two independent experiments. B. [3H]thymidine incorporation was assessed in cells stimulated with anti-CD3/anti-CD28 mAb-coated beads, in the presence or absence of 2-DG. Data represent the average + SD of three independent experiments and the inhibition by 2-DG was statistically significant (p<0.001). C. CFSE-labeled 2C CD8+ effector T cells were stimulated with P815-B7.1 cells in the presence or absence of 10 mM 2-DG. After 48 hours, dilution of CFSE was analyzed by flow cytometry. D. DNA content was assessed of effector T cells stimulated for 48 hours with or without 10 mM 2-DG.