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. Author manuscript; available in PMC: 2010 Dec 22.
Published in final edited form as: Eur J Immunol. 2008 Sep;38(9):2438–2450. doi: 10.1002/eji.200838289

Figure 7. Oxygen is not a main energy source for CD8+ effector T cells.

Figure 7

A. The rate of O2 consumption of 3 × 107 unstimulated CD8+ effector T cells. A reduced rate of consumption by the addition of rotenone (5 μM) shows that O2 consumption is mediated via mitochondria. The addition of N, N, N′, N′-tetramethyl-p-phenylenediamine (TMPD; 200 μM) and ascorbate (A; 400 μM) reflects the maximum potential for O2 consumption by these cells. Data are representative of two independent experiments. B. Steady-state levels of ATP in CD8+ effector T cells stimulated with anti-CD3 and anti-CD28 coated beads for 3 hours under the indicated conditions. Control (ctrl), 21% O2, 5% CO2; hypoxia (hyp), 1% O2, 5% CO2; 2-DG (2-DG), 50 mM 2-DG, 21% O2, 5% CO2. Inhibition by 2-DG was statistically significant (p<0.001). Data represent the average + SD of three independent experiments.