FIG. 5.
Clamping effects of PNA on PCR. Delays in the CT with different concentrations of complementary (solid lines) and noncomplementary (dashed lines) PNAs were measured with the MGB probe for the LLAQ strain (filled circles) and the LMAQ strain (open triangles). PNAs used were complementary (A) or noncomplementary (B) to the LLAQ strain and complementary (C) or noncomplementary (D) to the LMAQ strain. A delay in the CT was observed even with noncomplementary PNAs when the concentration was high. The differences in delays between PNAs complementary and noncomplementary to the LLAQ strain are similar to those between PNAs complementary and noncomplementary to the LMAQ strain.