PFGE patterns of chromosomal SmaI digests of Sp6C isolated from children in southern Israel from 1999 to 2008. Strains marked by asterisks were characterized by MLST (Table 1). Lanes 1 to 7, ST3531; lanes 8 to 12, ST1692; lanes 13 and 14, ST4387; lanes 15 and 16, ST367; lanes 17 and 18, ST4386; lanes 19 and 20, ST4439; lane 21, ST4440; lane 22, ST4441; lane 23, ST4381; lane 24, ST4442; lane 25, ST4438; lane 26, ST123; lane 27, ST4601. λ, lambda ladder; R6, S. pneumoniae reference strain used as a molecular weight marker.