Multiple sequence alignments of the amplified part of the bacterial 16S rRNA gene. Sequences of the bacteria included in the multiprobe assay are shown. The arrows represent the forward and reverse primer targeting the 16S rRNA gene. The sequences of the probes are in boldface and underlined. a, Staphylococcus probe; b, Enterococcus probe; c, Pseudomonas probe; d, S. aureus probe; e, E. coli probe; f, Streptococcus probe; g, universal probe; h, S. pneumoniae probe; i, P. aeruginosa probe.