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. 2010 Oct 20;48(12):4432–4438. doi: 10.1128/JCM.00562-10

FIG. 2.

FIG. 2.

Partial sequence alignments of the 16S rRNA gene, derived from the clinical isolates with discordant results. (A) Region where the probe specific for S. aureus binds. The sequences of the eight discrepancies are shown in the rectangle. The nucleotides underlined in the rectangle represent the nucleotides which distinguish S. aureus from Staphylococcus (Staph) spp. The sequence alignment of clinical isolate (CI) 7 is the exact reverse complement of the S. aureus probe sequence, which implicates that this isolate, which was identified as CoNS, is in fact S. aureus. CI 1 was not shown because the sequence could not be determined. GenBank accession numbers of reference strains are as follows: S. aureus, FN433596; Staphylococcus spp., GQ222246; and S. epidermidis, GQ911565. (B) Region where the probe specific for Enterococcus spp. binds. The nucleotides underlined in the rectangle represent the nucleotides which distinguish Enterococcus spp. from A. viridans. Conventional testing identified CI 10 as Aerococcus viridans, whereas our assay and sequencing classified it as an Enterococcus sp. GenBank accession numbers of reference strains are as follows: Enterococcus spp., AB489105; A. viridans, M58797. *, reference sequences.