TABLE 1.
Probes designed for use in multiplex PCR
| Probe | Sequence (5′-3′)a |
|---|---|
| Pseudomonas species | NED-CCTTCCTCCCAACTTAAAGTGCTT-MGB |
| P. aeruginosa | JOE-CCAAAACTACTGAGCTAGAGTACG-BHQ1 |
| E. coli | JOE-GGAGTAAAGTTAATACCTTTGCTCATT-BHQ1 |
| Staphylococcus species | NED-AATCTTCCGCAATGGGCGAAAGC-MGB |
| S. aureus | FAM-AGATGTGCACAGTTACTTACACATAT-BHQ1 |
| Enterococcus species | JOE-TCCTTGTTCTTCTCTAACAACAGAG-BHQ1 |
| Streptococcus species | NED-CCAGAAAGGGACSGCTAACT-MGB |
| S. pneumoniae | JOE-CCAAAGCCTACTATGGTTAAGCCA-BHQ1 |
a
NED, fluorescent label (Applied Biosystems); MGB, minor groove binder; JOE, 6-carboxy-4,5-dichloro-2,7-dimethyoxyfluorescein; BHQ1, black hole quencher 1; FAM, 6-carboxyfluorescein.