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. 2010 Sep 29;48(12):4592–4594. doi: 10.1128/JCM.01765-10

FIG. 2.

FIG. 2.

(a) Example of an agarose gel showing Acinetobacter isolates for which the species were determined by multiplex PCR using gyrB-directed primers D14, D19, D16, and D8. Lanes: 1, 100-bp marker; 2 and 3, A. calcoaceticus; 4 and 5, GS3; 6, A. baumannii; 7, GS13TU; 8, pooled DNA from A. lwoffii, A. junii, A. haemolyticus, and A. johnsonii. (b) Example of an agarose gel showing Acinetobacter isolates for which the species were determined by PCR using gyrB-directed multiplex primers (D14, D19, D16, D8, Sp2F, Sp4F, and Sp4R). Lanes: 1, 100-bp marker; 2, A. calcoaceticus; 3, A. baumannii; 4, GS3; 5, GS13TU; 6, pooled DNA from A. lwoffii, A. junii, A. haemolyticus, and A. johnsonii. In this gel, GS3 and GS13TU amplify an additional 1,194-bp product.