TABLE 1.
Morphological group, number of gene clones and genotypes of Acanthamoeba from freshwater sources and clinical isolates
| Isolate | Source/condition of the sample during collection | Groupa | No. of haplotypesb |
Genotype | Growth at °Cc |
|||
|---|---|---|---|---|---|---|---|---|
| Rns | DF3 | 25-30 | 37 | 40 | ||||
| AE1 | Lampangpui pond, Bangkok/29.5°C, pH 8.0 | I | 3 | 2 | T17 | + | − | − |
| AE2 | Lampangpui pond, Bangkok/28.5°C, pH 7.4 | I | 3 | 2 | T9 | + | − | − |
| AE3 | Freshwater pond, Kasetsart University, Bangkok/31.5°C, pH 7.9 | II | 1 | 1 | T4 | + | + | + |
| AE4 | Freshwater pond, Kasetsart University, Bangkok/30°C, pH 7.3 | II | 5 | 3 | T4 | + | + | + |
| AE5 | Muangthong Thani lake, Nonthaburi/34°C, pH 9.4 | II | 2 | 1 | T4 | + | + | + |
| AE6 | Rangsit distribution canal, Pathumthani/24°C, pH 8.0 | II | 5 | 2 | T4 | + | + | + |
| AE7 | Freshwater pond at National Stadium, Bangkok/32.5°C, pH 8.0 | II | 2 | 1 | T4 | + | + | + |
| AE8 | Freshwater pond at National Stadium, Bangkok/34.5°C, pH 8.2 | II | 3 | 1 | T4 | + | + | + |
| AE9 | Freshwater pond at National Stadium, Bangkok/32°C, pH 8.0 | I | 2 | 1 | T17 | + | − | − |
| AE10 | Freshwater pond at National Stadium, Bangkok/28.5°C, pH 7.7 | II | 3 | 1 | T4 | + | + | + |
| AE11 | Sammakorn pond, Bangkok/32°C, pH 8.2 | II | 1 | 1 | T4 | + | + | + |
| AE12 | Sammakorn pond, Bangkok/30°C, pH 8.0 | II | 4 | 1 | T4 | + | + | + |
| AE13 | Sammakorn pond, Bangkok/28°C, pH 8.5 | II | 1 | 1 | T3 | + | − | − |
| AE14 | Lampangpui pond, Bangkok/28.5°C, pH 8.3 | II | 5 | 4 | T4 | + | + | + |
| AE15 | Lampangpui pond, Bangkok/30°C, pH 7.8 | II | 1 | 1 | T4 | + | + | + |
| AE16 | Perfect Place pond, Bangkok/29°C, pH 8.3 | II | 1 | 1 | T4 | + | + | + |
| APC1′ | Corneal scrape, 16-year-old female/keratitis, contact lens user | II | 1 | 1 | T4 | + | + | + |
| APC2 | Corneal scrape, 44-yr-old male/keratitis, glaucoma | III | 1 | 1 | T10 | + | + | + |
| APC3 | Corneal scrape, 31-yr-old female/keratitis, contact lens user | II | 4 | 2 | T4 | + | + | + |
| APC4 | Corneal scrape, 42-yr-old female/keratitis, exposed to fish farm water | II | 3 | 1 | T4 | + | + | + |
| APC5 | Corneal scrape, 21-yr-old female/keratitis, contact lens user | II | 1 | 1 | T4 | + | + | + |
| APB1 | Cerebrospinal fluid, 31-yr-old female/meningoencephalitis | II | 1 | 1 | T4 | + | + | + |
a
Pussard and Pons' morphological group (12).
b
Number of haplotypes based on near-complete Rns sequences (Rns) and diagnostic fragment 3 (DF3).
c
Growth determined within 10 days of incubation. +, growth; −, absence of growth.