TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Genotype or phenotype | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK+) supE44 λ−thi-1 gyrA relA1 | 13 |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm met (DE3) | Novagen |
| C41(DE3) | Derived from BL21(DE3); at least one uncharacterized mutation | 32 |
| C43(DE3) | Derived from C41(DE3); at least two uncharacterized mutations | 32 |
| Allochromatium vinosum | ||
| Rif 50 | Rifr, spontaneous rifampin-resistant mutant of A. vinosum DSMZ 180T | 25 |
| ΔdsrM mutant | Rifr, in-frame deletion in dsrM | 42 |
| ΔdsrK mutant | Rifr, in-frame deletion in dsrK | 42 |
| ΔdsrP mutant | Rifr, in-frame deletion in dsrP | 42 |
| ΔdsrJ+Jhis mutant | Rifr, Kanr, ΔdsrJ mutant carrying plasmid pBBRJHis | 10 |
| Plasmids | ||
| pET11a | Apr, T7 promoter | Novagen |
| pET22b | Apr, His tag, T7 promoter | Novagen |
| pPR-IBA-1 | Apr, Strep tag, T7 promoter | IBA |
| pASK-IBA-3 | Apr, Strep tag, Tet promoter | IBA |
| pMexC | Apr, His tag, NdeI-BamHI fragment of amplified dsrM in pET11a | This work |
| pPexC | Apr, His tag, NdeI-EcoRI fragment of amplified dsrP in pET22b | This work |
| IBAdsrK | Apr, Strep tag, EcoRI-Eco47III fragment of amplified dsrK in pPR-IBA-1 | This work |
| IBAdsrO | Apr, Strep tag, EcoRI-XhoI fragment of amplified dsrO in pASK-IBA-3 | This work |