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Electrophoretic analysis of bacterial proteins. Proteins present in E. coli BL21 lysates and chromatographic fractions were size fractionated on 15% polyacrylamide gels under denaturing conditions and visualized by staining with Coomassie blue. Lanes: 1, molecular mass markers; 2, cell lysate from uninduced cells; 3, cell lysate from induced cells; 4, NiNTA-agarose chromatographic flowthrough fraction; 5, NiNTA-agarose chromatographic fraction isolated with elution buffer.
