FIG. 5.

Effects of light on biofilm formation. The biofilms formed by parental strain ATCC 17978 (A) and the ATCC 17978.OR blsA mutant harboring no additional plasmid (B) or transformed with the blsA-complementing derivative pWHBLSA (C) or the shuttle vector pWH1266 (D) were recorded after static incubation for 96 h at 24°C by direct visual inspection and staining with crystal violet. The white arrows point to the pellicles formed on the liquid surface. (E) Quantification of total biofilms (pellicles plus cells attached to tube walls) of duplicate samples shown in the cognate panels. Error bars show the standard error of the mean.