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. 2010 Oct 15;192(24):6346–6351. doi: 10.1128/JB.00838-10

FIG. 1.

FIG. 1.

Structure of B. abortus GI-2 and the corresponding B. ovis locus. (A) Genetic organization of B. abortus 2308 15.1-kb GI-2. Gray arrows, phage-related genes (int, integrase gene); cross-hatched arrows, LPS biosynthesis genes; white arrows, unknown or hypothetical ORFs; black arrow, IS711 (containing the transposase ORF); white vertical bars, tRNA genes; black boxes (within the tRNA gene or a hypothetical ORF flanking the GI), direct repeats sequences. The positions of primers BAB1_0979F and BAB1_1008R (used to amplify a 17.3-kb fragment, including GI-2) are marked P1 and P2, and the positions of primers BAB1_0983F and -R (used to amplify a 401-bp fragment of the integrase) are marked P3 and P4, respectively. The ClaI-ClaI segment indicates the position of the corresponding IS711-carrying 4.2-kb restriction fragment. (B) Genetic organization of the B. ovis ATCC 25840 locus carrying the GI-2 excision scar. In this case, primers P1 and P2 amplify a 2.2-kb fragment spanning the attB-containing scar (black box) and a PvuII site. The positions of primers BAB1_0981F and BAB1_1007R (used to amplify a 586-bp internal fragment of the scar) are marked P5 and P6, respectively.