TABLE 1.

Spontaneous suppressor mutations that accumulated in ΔackA mutants

Phenotype group and mutated gene	Mutation in independent isolates
Mucoid large colony suppressors
spxB	Phe269Leu (TTT to CTT)
spxB	Frameshift mutation ΔC in codon 264
spxB	Gly472Asp (GGC to GAC)
spxB	Change in mapped transcription start from G to T
Rough medium-sized colony suppressors
spxB	Trp339STOP (TGG to TAG)
cps2Ea	Frameshift mutation ΔA in codon 326 (in run of 7 As)
spxB	Gly423Cys (GGT to TGT)
cps2Ea	Frameshift mutation ΔA in codon 326 (in run of 7 As)
spxR	Frameshift mutation ΔG in codon 115 (in run of 5 Gs)
cps2Ea	Frameshift mutation ΔA in codon 326 (in run of 7 As)
Additional capsule mutations identified in rough medium-sized colony suppressors
cps2E	Frameshift mutation ΔG in codon 111
cps2E	Arg347Cys (CGT to TGT)
cps2E	Frameshift mutation ΔA in codon 419
cps2E	Insertion of T after codon 204
cps2C	Insertion of A after codon 45
cps2C	Insertion of T in codon 113

^aIsolated three times from separate independent transformations.