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. Author manuscript; available in PMC: 2010 Dec 22.
Published in final edited form as: Nat Genet. 2007 Jan 28;39(3):380–385. doi: 10.1038/ng1969

Figure 1.

Figure 1

Dgcr8 knockout strategy. The DGCR8 protein has one WW domain and two double-stranded RNA-binding domains (dsRBDs). The WW domain is encoded in exon 4 of the Dgcr8 genomic DNA (gDNA); the two dsRBDs are located in exons 7–9 and 10–12, respectively. Excision of exon 3 from the mRNA generates a frame shift, resulting in several premature stop codons in downstream exons. ES cells were sequentially targeted and treated with Cre recombinase to produce Dgcr8 Δ/flox and Δ/Δ ES cells. Rescue cells were generated by retargeting Δ/Δ ES cells with the 3lox construct, followed by removal of the HygroTK cassette with Cre recombinase.