Figure 2.

Effect of the guanylate cyclase inhibitor ODQ (10 μM) and the PKG inhibitor RP-8CPT-cGMPs (5 μM) on NO induction of ecSOD expression in HASMs. HASMs were exposed to DETA-NO (100 μM) for 12 hours in the absence or presence of the various antagonists noted. (a and c) Representative RNase protection assays. (b and d) Mean data quantified by densitometry of immunoblots. The data are expressed as fold increases in ecSOD expression. Values are the mean ± SE for three separate experiments. ^AP < 0.01 versus control.