Figure 4.

Evidence for a role of p38MAP kinase in modulation of ecSOD expression by NO. HASMs were exposed to DETA-NO (100 μM) for 12 hours in the absence or presence of the various antagonists noted (a and b), or for 60 minutes (c). (a) A representative RNase protection assay. (b) Mean values of densitometric analysis. (c) A representative Western analysis for p38MAP kinase phosphorylation by 100 μM DETA-NO (n = 3). HASMs were treated with DETA-NO for 1 hour before harvest. Western analysis was performed using a specific anti-phospho p38MAP kinase (p38MAPK-P) antibody. Values are the mean ± SE for three separate experiments. ^AP < 0.01 versus control cells.