Figure 5.

Effect of DETA-NO (100 μM) on ecSOD transcription rate and mRNA stability in HASMs. For nuclear-run on studies, nuclei were harvested from HASMs under control conditions and after 4 hours of exposure to DETA-NO. For mRNA stability, HASMs were exposed to either control conditions or DETA-NO for 10 hours and then exposed to actinomycin D (10 μg/mL). Messenger RNA levels were determined using RNase protection assays as shown in Figures 5–8. For both panels, the left portion shows representative experiment, whereas the right panel shows mean data for three (a) and four (b) separate experiments. Mean data in b are expressed as a percent of mRNA at time 0. ^AP < 0.05 versus control cells. NS, not significant.