Figure 7.

Total SOD activity and activities of individual SOD subtypes in response to DETA-NO (100 μM) on mouse aortas (C57BL/6) in organoid culture. Aortas were homogenized, and SOD activity was assayed by examining inhibition cytochrome c reduction by xanthine/xanthine oxidase at pH 7.4. The activity of MnSOD was examined by addition of KCN (3 mM). The activity of ecSOD was determined after separation with Con A-Sepharose. Experiments were performed on four pooled aortas on three separate occasions. The results are presented as mean ± SEM, and the values are expressed as units per milligram of total protein. ^AP < 0.05 versus control cells.