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. 2010 Dec 22;5(12):e15047. doi: 10.1371/journal.pone.0015047

Figure 6. Mutation of the EGR-1 binding site abolishes the GFP transient expression due to the 5.0 Kb construct.

Figure 6

The nature of the injected construct is indicated in the stripes at the top of each figure (-5.0prep1.1:GFP or -5.0prep1.1mutEGR:GFP). Lateral views of 30 hpf embryos are shown. Below each figure, the sequence of the wild type or of the mutated (in red) EGR-1 binding site is reported. In the most of cases (see also Table 4 ) the mutation causes the complete abolishment of GFP expression.