Skip to main content
. 2010 Dec 22;5(12):e15047. doi: 10.1371/journal.pone.0015047

Table 4. List of the oligonucleotides used to clone 5-untranscribed regions of prep1.1.

Oligonucleotide
humanF100 bp 5′-cgaggcaaatggtttcgcccg-3′
humanF700 bp 5′-ctgcagtattgaaggccagtcagg-3′
humanF1800 bp 5′-ccaaagaatggctactccataggcag-3′
humanF5000 bp 5′-gcaattctcatgcctctgtgataccc-3′
humanR(for every construct) 5′-gcagagcctcccgccttatgac-3′
zebrafishF100 bp 5′-ggacagaacaccagagaactaattagc-3′
zebrafishF700 bp 5′-cttatgttacttgcgttaccctcaatactg-3′
zebrafishF1800 bp 5′-gcctttactgcatggcctgtgtacattttg-3′
zebrafishF5000 bp 5′-gcattatgcagtgagtctagctccac-3′
zebrafishR(for every construct) 5′-cggggaggagaagtgacgtgatgc-3′
humanFint 5′-gtaagctgtcactcaaccgctcc-3′
humanRint 5′-catgcgggccggtgtgattg-3′
zebrafishFint 5′-gtaagctgtcactcaaactcgtc-3′
zebrafishRint 5′-gggagtaaatgtagcagcggtctc-3′

F stands for forward; R for reverse.