Table 4. List of the oligonucleotides used to clone 5-untranscribed regions of prep1.1.
Oligonucleotide | |
humanF100 bp | 5′-cgaggcaaatggtttcgcccg-3′ |
humanF700 bp | 5′-ctgcagtattgaaggccagtcagg-3′ |
humanF1800 bp | 5′-ccaaagaatggctactccataggcag-3′ |
humanF5000 bp | 5′-gcaattctcatgcctctgtgataccc-3′ |
humanR(for every construct) | 5′-gcagagcctcccgccttatgac-3′ |
zebrafishF100 bp | 5′-ggacagaacaccagagaactaattagc-3′ |
zebrafishF700 bp | 5′-cttatgttacttgcgttaccctcaatactg-3′ |
zebrafishF1800 bp | 5′-gcctttactgcatggcctgtgtacattttg-3′ |
zebrafishF5000 bp | 5′-gcattatgcagtgagtctagctccac-3′ |
zebrafishR(for every construct) | 5′-cggggaggagaagtgacgtgatgc-3′ |
humanFint | 5′-gtaagctgtcactcaaccgctcc-3′ |
humanRint | 5′-catgcgggccggtgtgattg-3′ |
zebrafishFint | 5′-gtaagctgtcactcaaactcgtc-3′ |
zebrafishRint | 5′-gggagtaaatgtagcagcggtctc-3′ |
F stands for forward; R for reverse.