Figure 5. Mutant Ppt1 and Ppt1 RNAi knock-down in neurons result in defective axon tracts in the developing CNS.

In all panels, S17 embryos were stained with 1D4 antibody. (A) FASII is expressed in three longitudinal connectives at this stage. (B) Ppt1^A179T embryos exhibit discontinuous and frayed longitudinal connectives. (C, D) Ppt1 RNAi expression under the control of elav gal4 driver results in a range of axon guidance phenotype including wavy bundles with some faulty axon crossing over the midline (arrow in C); and disorganized and/or broken connectives, and fused commissures (arrows in D). Anterior, up.