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. 2010 Dec 22;5(12):e15577. doi: 10.1371/journal.pone.0015577

Figure 1. Knock-down analysis of TbUNC119.

Figure 1

A. Growth of TbUNC119 knock-down procyclic form cells. Three TbUNC119 knock-down inducible cell lines were cloned (TbUNC119-1, TbUNC119-2 and TbUNC119-3). RNAi-mediated gene knock-down was induced with tetracycline. (Tet+) denotes RNAi-induced cells. There was no significant difference in the growth of the cell lines with or without RNAi induction. The inset shows TbUNC119 RT-PCR results with tubulin as a control. B. Motility assay of TbUNC119 knock-down cells. TbUNC119 knock-down cells and parental 29-13 cells were assayed for motility 7 days after RNAi induction or without RNAi induction. Thirty seconds of motion was captured for each cell. Motility traces for 50 cells in each group were generated using Videopoint 2.5 software. Cells that were not tracked for the full 30 s were not used for the analysis. There was no significant difference in the motility of the cell lines with or without RNAi induction.