Table 2. Responses to VLP vaccination in ferrets.
| Experiment | Pre-challenge HI GMTa (fraction of vaccine responders) | Mean viral loadb (number of positive animals) | Mean % body weight change | Survival | ||
| Indo/5/05 | VN/1203/04 | URT | lungs | |||
| After 2nd dose | ||||||
| 1.8 µg VLP | 429 (8/8) | 24 (6/8) | 3.5 (1/3)c | 2.0 (2/3) | +2.1 | 5/5 |
| 3.7 µg VLP | 382 (8/8) | 28 (7/8) | 2.5 (2/3)c | <1.5 (0/3) | −0.3 | 5/5 |
| PBS | <8 (0/8) | <8 (0/8) | 3.2 (3/3) | 2.25 (1/3) | −17.7 | 0/5 |
a
HI titers measured on sera taken 14 days after last vaccination.
b
Values are expressed as log10EID50/ml, mean calculated on positive animals only. Individual values by dose and location: 1.8 µg URT (3.5, < LOD,< LOD) lungs (1.98, 2.0, <LOD); 3.7 µg URT (2.5, 2.5, < LOD) lung (< LOD, < LOD,< LOD), placebo URT (3.75, 3.33, 2.5) lungs (2.25, 2 x< LOD).
c
Virus titration performed on animals sacrificed 3 days post-challenge.