Figure 3. Cfl1 MOs efficiently block the translation of GFP fusion constructs containing respective MO binding site.

Embryos at 1-cell stage were injected with 330 pg pCS2+ XLT fused with a fragment of cfl1 gene sequence from −22 to +195 or −52 to +51 in the absence or presence of 7.5 ng cfl1 tMO₁ or tMO₂, respectively. The expression of GFP was examined at 10 hpf and photographed under epifluorescent microscopy. The representative photos for embryos treated without cfl1 MO (A) and with tMO₁ (B) are shown and the percentages of injected embryos expressing GFP are shown in average ± standard deviation (C).