Figure 10. Knockdown of cfl1 inhibits the pseudopod formation of involuting cells.

Embryos injected with MOs were dechorionated, immobilized, monitored, and recorded in 15-min continuous time-lapse movies. Lateral cells were monitored from the lateral side (A, C), and prechordal plate cells were monitored from the anterior side (B, D). Representative photographs are shown for each group as designated. Arrows indicate the sites of pseudopods. (E) Number of pseudopods was counted and analyzed for each group. Both lateral cells and prechordal plate cells in cfl1 morphant embryos had significantly fewer pseudopods (* p<0.05). Only upper error bars of the standard deviations are shown.