Fig. 4.

Comparative analysis of HSCCC chromatogram of 50 mg of the crude sample at different column volumes: (A) 125 mL capacity (B) 250 mL capacity (C) 375 mL capacity (D) 500 mL capacity. Solvent systems: n-hexane–ethyl acetate–methanol–water (7.5:15:6:7, v/v). Note: The apparatus was equipped with four PTFE preparative coils (1.6 mm ID tubing, each 125 mL capacity with a total capacity 500 mL), symmetrically mounted on the rotary frame. Four preparative coils can be operated in parallel or series or as individual coil.