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. 2010 Sep 27;588(Pt 22):4519–4537. doi: 10.1113/jphysiol.2010.196618

Figure 10. PLA detection of Kv subunit proteins in adjacent channel complexes in HEK 293 cells and RMCA myocytes.

Figure 10

A, PLA signals were detected at the periphery of GFP-positive cells transfected with Kv1.2 and Kv2.1, but not in GFP-negative cells probed with Kv1.2 and Kv2.1 primary antibodies. B, PLA signals were not detected at the periphery of GFP-positive cells transfected with Kv1.5 and Kv9.3 and probed with Kv1.5 and Kv9.3 primary antibodies. C, PLA signals were detected at the periphery of GFP-positive cells transfected with Kv1.5, Kv9.3 and Kv2.1, but not in GFP-negative cells probed with Kv1.5 and Kv9.3 primary antibodies. D, PLA signals were not detected at cell periphery of GFP-positive cells transfected with Kv1.5 and Kv2.1 and probed with Kv1.5 and Kv9.3 primary antibodies. E, representative RMCA myocyte exhibiting PLA signals at cell periphery when probed with Kv1.5 and Kv9.3 primary antibodies. F, representative RMCA myocyte exhibiting PLA signals at cell periphery when probed with Kv1.2 and Kv2.1 primary antibodies.