Figure 2. Increases in unstimulated [Ca²⁺]_i were less during a second fatigue bout (FAT2) than during a first bout (FAT1) in the presence of glibenclamide.

Fatigue protocols were as described in Fig. 1A. FAT1 (○) and FAT2 (•) were elicited under control conditions (Con) (A) or in the presence of 10 μm glibenclamide (Gli) (B). Unstimulated [Ca²⁺]_i is defined as the [Ca²⁺]_i just before a contraction was elicited. Vertical bars represent the s.e.m. of 11 fibres for all conditions except for FAT1-Gli with 19 fibres. Horizontal bars (with numbers indicating the fatigue bout number) represent the time period when mean unstimulated [Ca²⁺]_i was significantly different from the value at time 0 s; *mean values between FAT1 and FAT2 were significantly different. ANOVA and LSD, P < 0.05.