Figure 9. Blocking mK_ATP channels with 5-HD during FAT1 did not prevent FPC.

A, fatigue protocol. Paired FDB bundles were used. For one bundle (protocol a), FAT1 was elicited in control conditions (FAT1-Con) and FAT2 in the presence of 10 μm glibenclamide (FAT2-Con/Gli). For the paired bundle (protocol b), FAT1 was elicited in the presence of 300 μm 5-HD (FAT1-5-HD) and FAT2 in the absence of 5-HD but in the presence of 10 μm glibenclamide (FAT2-5-HD/Gli). 5-HD was added 30 min prior to FAT1 and removed 30 min after FAT1. B and C, tetanic force (B) and unstimulated force (C) are expressed as a percentage of the pre-FAT1 tetanic force. Data for FAT1-Con are not shown for clarity. Data for FAT1-Gli of Fig. 2A are shown for comparison. Vertical bars represent the s.e.m. of 5 FDB bundles. *Tetanic force and unstimulated force significantly different from FAT2, ANOVA and LSD, P < 0.05.