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. 2010 Oct 19;4(1):120–125. doi: 10.1242/dmm.006114

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Fig. 3. — Localization of Btn1p is dependent upon the pH of the growth media. (A) Yeast co-expressing Btn1p-eSapphire and Vma2p-GFP grown for 6 hours in SC-URA at pH 6.0 clearly showing that Btn1p predominantly co-localizes with Vma2p in the vacuolar membrane. (B) Same strain grown in SC-URA at pH 4.0 for 6 hours showing that Btn1p is not in the vacuolar membrane but predominantly in unidentified puncta. (C) Vacuolar enrichment from strains expressing Btn1p-V5 grown at pH 4.0 and 6.0 showing that Btn1p co-fractionates with the vacuolar membrane protein Vph1p after growth at pH 6.0 but not at pH 4.0. (D) Vacuoles from btn1-Δ cells expressing Btn1p-N175Q, N178Q, N206Q-V5 (all three putative N-glycosylation sites mutated).