Fig. 1.

Ena localises to the tips of filopodia and the leading edge of lamellipodia in vivo. Haemocytes co-expressing Ena-GFP (green) and mCherry-Moesin (red) were imaged at stage 15 of embryonic development. (A) Ena-GFP localises to the tips of filopodia (arrowhead) and the leading edge of lamellipodia (bracket) in haemocytes at the midline. (B) Ena-GFP at a filopodial tip during extension and retraction (see supplementary material Movie 1 for an example of Ena-GFP being lost during retraction). (C′) Kymograph generated along the axis of protrusion (solid line in C), demonstrating the presence of Ena-GFP at the leading edge during lamellipodial extension and its loss from this site upon retraction (arrowheads); the dotted line indicates the time point in the kymograph corresponding to the still image in C. (D) Ena-GFP is present at the lamellipodial leading edge during lateral migration (see supplementary material Movie 2 for corresponding time-lapse). Dots and lines reveal progress of the haemocyte when moving from the midline to the edge of the VNC. (E) Images showing the GFP channel of the haemocyte from D; in these images Ena-GFP is clearly localised at the lamellipodial leading edge (arrowheads). Scale bars in C’ represent 2 μm (vertical) and 60 seconds (horizontal); all other scale bars represent 10 μm.