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Competitive binding of anti-CD58 and FITC-P4. The concentration of anti-CD58 was kept constant (6.25 µg/mL) and the concentration of FITC-P4 was varied. For comparison, 100 µM of FITC-P4 in the absence of antibody (−) is also shown. P < 0.05 for 50 µM FITC P4 when compared to 100 µM in the absence of antibody. P >0.05 for 200 and 100 µM FITC P4 in the presence of antibody when compared to 100 µM in the absence of antibody.
