Figure 1.

Activity of rHPLC6 and mutant constructs. (A) Ice crystal morphology of the four proteins in the thermal hysteretic gap. The identity of the protein is indicated below each photograph. The scale bar is the equivalent of 25 μm in length for all photographs. (B) Thermal hysteresis measurements. The activities of the constructs were measured using a nanoliter osmometer as described in the Material and Methods section. Synthetic HPLC6, open square; rHPLC6, open circle; rHPLC6-Ala³⁷-NH₂, open triangle; rHPLC6-Arg³⁷, solid circle; rHPLC6-Ala³⁷, solid triangle. The lines indicate the average trend for each of the proteins. Synthetic HPLC6 and rHPLC6, solid line; rHPLC6-Ala³⁷-NH₂, dotted and dashed line; rHPLC6-Arg³⁷, dashed line; rHPLC6-Ala³⁷, dotted line. The error bars represent the standard deviation of three independent measurements.