Table III.

Complementation Analysis and Specific Activity of Wild Type and Mutant Recombinant His-tag KdsD Proteins

		Growth conditiona
Complementing plasmid	Codon change	+ Ara	+ Glu	Protein	Specific activityb (mU/mg)	Residual activityc
pRSETb	—	+	—d			—
pRSETb/kdsD	—	+	+	KdsD	29.55 ± 0.89	100
pRSETb/kdsDH88A	CAT → GCT	+	+	KdsDH88A	2.81 ± 0.19	9.5
pRSETb/kdsDK59A	AAA → GCA	+	—d	KdsDK59A	0.72 ± 0.06e	2.4
pRSETb/kdsD1–183	—	+	—d	KdsD1–183	nd	nd

nd: not determined.

^aLD-ampicillin agar plates supplemented with Ara (+ Ara) and Glu (+ Glu) for induction and repression, respectively, of BB-8 chromosomal kdsD gene.

^bSpecific activity was determined as reported in Materials and Methods. Each measurement is the mean of at least five independent determinations. Standard deviations never exceeded 10%.

^cPercentage (%)-specific activity in comparison with the wild-type protein.

^dEfficiency of plating ≤10⁻⁴.

^eData from Sommaruga et al.¹⁷