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Six fusion vectors used in HTP systems. Cloning and expression regions of the six expression vectors and corresponding insertion locations of target proteins were used in this work. Recombinant fusion vectors were named by six fusion tags; i.e., (A) calmodulin-binding peptide (CBP), (B) glutathione S-transferase (GST), (C) N utilization substance A (NusA), (D) Histidine (His), (E) maltose-binding protein (MBP), and (F) thioredoxin (Trx).
