Figure 4.

nonmonotone nonlinearity in Kluveromycetes lactis. We experimentally observed that the synthesis of using galactose in Kluveromycetes lactis exhibits the shown nonlinearity. WT culture was grown in different concentrations of lactose and galactose in liquid culture. The concentrations used were 0.1%, 0.5%, 1%, and 2%. The beta-galactosidase activity was measured in different concentrations of sugar by a protocol described by Miller method (see [20]). The maximum enzyme activities at different concentrations are plotted; the plots are not to scale.