Skip to main content
. Author manuscript; available in PMC: 2010 Dec 23.
Published in final edited form as: Ophthalmic Genet. 2010 Mar;31(1):24–29. doi: 10.3109/13816810903426249

FIGURE 4.

FIGURE 4

(A) T cell proliferative response to phytohemaglutinin (PHA) and anti-TCR stimulation. Peripheral blood mononuclear cells (PBMCs) were isolated from fresh blood from the patient and a healthy donor. PBMCs were stimulated with PHA or anti-CD3 with without anti-CD28 at different dosages as depicted in the figure. Tritium labeled thymidine incorporation was used to measure proliferative response of T cells to the stimuli. There was no difference in T cell proliferation between the patient and a healthy control. (B) Increased CD28 negative T cells in patient compared to that in normal control. Whole blood samples were analyzed by a 4-color flow cytometry staining using FITC-CD4, PE-CD28, PE-CY7-CD3 and APC-CD8 antibodies (BD Biosciences, CA). The T cell staining was analyzed using FlowJo software (TriStar, San Jose, CA). CD28 negative T cells were gated based on CD3+CD4+CD28- and CD3+CD8+CD28- triple staining. CD28 negative T cells in both CD3+CD4+ and CD3+CD8+ T cells were much higher in DGS (DiGeorge syndrome) patient than in a normal control donor. A patient with a diagnosis of NK (natural killer cell) lymphocytosis and multiple autoimmune diseases also showed increased CD28 negative T cells.