Figure 3.

ET induces binding activity of NFκB p65 and p50 in SCp2 cells. SCp2 cells were treated as described in Methods with 0 or 10 μg of ET per ml in 1% FBS-DM for 0, 1, 3, and 6 h. Isolated nuclear proteins of control (0 μg ET) and ET-treated (10 μg ET) SCp2 cells were analyzed for (A) NFκB p65 or (B) NFB p50 binding activities by immunobinding assays, with relative binding activity shown as A₆₉₀ above blank. Solid-line and closed symbol represent the results of control non ET-treated samples, while dashed-line and open symbol represent the results of samples from ET-treated cells. This experiment was performed at least twice. Data represents the average for duplicate samples ± SD of a representative experiment. (*) denotes significant difference among treatment within each time point (p < 0.05).