Figure 3.

Western blot analysis of talin. Upper panel: A representative western blot for the analysis of intact and cleaved talin (BDP) in the diaphragm of controls (C, n = 6) and controlled mechanical ventilation with saline injection (CMV, n = 9) or with 5 mg/kg methylprednisolone (MP-5, n = 7) or 30 mg/kg methylprednisolone (MP-30, n = 5). Lower panel: Mean densitometric values for talin degradation by calpain. Values are shown as the ratio of degraded talin to intact protein and are expressed as means ± SD, *p < 0.05 vs others.