Figure 12.

The figure shows volume fractions of Blochmannia symbionts in the midgut tissue of the various developmental stages shown in Fig. 1 to Fig. 10 calculated from the confocal image stacks as described in the Methods section in arbitrary units. The results show the strong relative decrease of Blochmannia-bearing midgut cells between L1 and L2, the strong increase in bacteria-infected cells during the P1 stage and the decrease of bacteria-infected cells in adult animals. Standard deviations are shown as vertical bars on top of the columns. Groups differing significantly at the p < 0.05 level in a Tukey HSD post hoc test are marked with different letters above bars. * W3-1 was not included in the statistical analysis due to small sample size.