Figure 4. mRNA export is impaired in mos11 plants.

(A) Morphology of 4-week-old soil-grown WT, mos11-1 and mos11-2 plants. (B) Dot blot of WT, mos11-1 and mos11-2. The same amount of WT, mos11-1, and mos11-2 total RNA were applied to the Hybond-N+ membrane and hybridized by ³²P-ATP labeled 18-mer oligo dT. The amount of total RNA loaded to each dot was indicated on the right of the blot. The radioactive signal was detected by a phosphor-imager. (C) Whole mount in situ mRNA localization of 7-day-old seedlings probed with 48-mer oligo d(T) labeled with Alexa 488. Observations were done at 63× magnification and microscope settings were identical for all samples.