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. 2010 Dec 23;6(12):e1001255. doi: 10.1371/journal.pgen.1001255

Figure 3. Generation of null mutants for each kep1 family gene and the male fertility test.

Figure 3

(A) Schematic maps of mutant alleles of kep1 family genes generated by P-element excision or targeted mutagenesis. The exons (black block), start codon (ATG), stop codon (TAA/TGA/TAG), and deleted genomic regions are indicated. For kep1 and CG4021, the P-element insertion for further excision is shown by a triangle, with a Bloomington stock number given underneath. (B) RT-PCR examination of null mutants (Null) for each kep1 family gene relative to WT flies (WT). Negative control (NC) is the reaction without reverse transcriptase, and the expression of rp49 is used as internal control. (C) Fertility test for nsr WT and mutant males. nsr WT: WT controls with identical genetic background with nsr mutants; nsr -/-: homozygous nsr mutants; nsr -/CyO: heterozygous nsr mutants; nsr Rescue: flies with a copy of WT nsr transgene in the nsr mutant background. Error bars indicate standard deviation. (D) Fertility test for kep1, CG3927, and CG4021 WT and mutant (-/-) males. Error bars indicate standard deviation.