Table 1.
Oligonucleotide primers used for PCR amplification of cDNAs obtained after reverse transcription from human testis
| Target (Accession number) | Sequence of primer (5'___ 3') | Tm (°C) | Amplified product (bp) |
|---|---|---|---|
| TGF-β1 (NM_011577) | F: TCCTGCTTCTCATGGCCA R: CCTCAGCTGCACTTGTAG |
55 | 405 |
| TGFBRII (NM_009371) | F: GCGCCAACAACATCAACC R: GGTACTCCTGCAGGTTGC |
56 | 300 |
| ALK-5 (NM_004612) | F: GACATCCCTGGTCCATCC R: TCTGGCACAACTCCACTG |
55 | 299 |
| ALK-1 (NM_000020) | F: GTCCTGGTTCCGGGAGA R: TTGCTCTTGACCAGCACA |
55 | 294 |
| Endoglin (NM_000118) | F: GCATCCTTCGTGGAGCTA R: GACACCTGCATGCCACA |
55 | 300 |
| β- Actin (AJ312092) | F: GGATGCAGAAGGAGATCA R: CTAGAAGCATTTGCGGTG |
55 | 193 |
F: forward; R: reverse