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. 2010 Dec 23;5(12):e14417. doi: 10.1371/journal.pone.0014417

Figure 1. Quantitative detection of Mp in mouse lung by real-time PCR.

Figure 1

A portion of the 16S rRNA (from nucleotides 201 to 265) that is specific for Mp was chosen as the molecular target for detection. Total RNA was purified from the lungs of one mouse, and RNA from the indicated number of Mp was added. 100 ng of total RNA was used for each real-time PCR reaction. Ct, cycle threshold. The sensitivity and linearity of the assay were confirmed in one additional experiment. Data shown are means ± SEM.