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. 2010 Dec 23;5(12):e15330. doi: 10.1371/journal.pone.0015330

Figure 3. WNT10A functions as an angio/stromagenetic growth factor in vivo xenograft models.

Figure 3

(A) Establishment of a stable WNT10A-overexpressing cell line. (B), (C) The growth rate of these stable cell lines (B) in vitro and (C) in vivo. Two control cell lines (cl:2; open circle, cl:3; open square) and two stable WNT10A-overexpressing cell lines (cl:6; closed circle, cl:25, closed square) were used. **P<0.01 compared with the control cl:2 group and #P<0.05 compared with the control cl:3 group using Scheffe's test. n = 8 per groups. (D) Representative photograph WNT10A-overexpressing tumors in nude mice illustrating their hypervascular nature. (E) Immunostaining of tumors with an anti-aSMA antibody. Increased numbers of aSMA+ cells (black arrows) are clearly visible in WNT10A-overexpressing tumors. (F) Reduced areas of tissue necrosis in WNT10A-overexpressing tumors are accompanied by increased tumor size and increased microvessel density (n = 4 or 6 per group, *P<0.05 and **P<0.01). Microvessel density was quantified using the number of aSMA+ cells (G) Masson trichrome staining showing expansion of the extracellular matrix and immunohistchemical analysis of mouse Type I collagen in the control and WNT10A-overexpressing tumors.